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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind especially to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to ensure the best possible benefits are...
Antibodies sure to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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